Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP1813c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1813c, RRID:AB_2062350
- Product name
- ATG7 Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line.
Energy restriction as an antitumor target of thiazolidinediones.
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.
Okamoto K, Sakimoto Y, Imai K, Senoo H, Shidoji Y
The Biochemical journal 2011 Nov 15;440(1):63-71
The Biochemical journal 2011 Nov 15;440(1):63-71
Energy restriction as an antitumor target of thiazolidinediones.
Wei S, Kulp SK, Chen CS
The Journal of biological chemistry 2010 Mar 26;285(13):9780-91
The Journal of biological chemistry 2010 Mar 26;285(13):9780-91
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Yang D, Liu H, Goga A, Kim S, Yuneva M, Bishop JM
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
Bigford GE, Alonso OF, Dietrich D, Keane RW
Journal of neurotrauma 2009 May;26(5):703-20
Journal of neurotrauma 2009 May;26(5):703-20
Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Ding ZB, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J
Cancer research 2008 Nov 15;68(22):9167-75
Cancer research 2008 Nov 15;68(22):9167-75
A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.
Xu ZX, Liang J, Haridas V, Gaikwad A, Connolly FP, Mills GB, Gutterman JU
Cell death and differentiation 2007 Nov;14(11):1948-57
Cell death and differentiation 2007 Nov;14(11):1948-57
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hAPG7L-R509 Pab (Cat. #AP1813c) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-R509(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-APG7L Pab (Cat. #AP1813c) is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- APG7L Antibody (R509) (Cat. #AP1813c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813c ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548)."
- Primary Ab dilution
- 1:200
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
