Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- AP1813D - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1813d, RRID:AB_2274542
- Product name
- ATG7 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Ding ZB, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J
Cancer research 2008 Nov 15;68(22):9167-75
Cancer research 2008 Nov 15;68(22):9167-75
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of APG7L (C-term) Pab (Cat. #AP1813d) in 293 cell line lysates transiently transfected with the ATG7 gene(2ug/lane).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of APG7L Pab (Cat. #AP1813d) in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term) (Cat. #AP1813d). U251 cells(right) were treated with Chloroquine (50 ¼M,16h). AP1813d was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue)."
- Primary Ab dilution
- 1:25
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100