MAB6608

antibody from Novus Biologicals

Targeting: ATG7 APG7L, DKFZp434N0735, GSA7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6608

Provider

Novus Biologicals

Product name

Mouse Monoclonal ATG7 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Autophagy drives fibroblast senescence through MTORC2 regulation.

Bernard M, Yang B, Migneault F, Turgeon J, Dieudé M, Olivier MA, Cardin GB, El-Diwany M, Underwood K, Rodier F, Hébert MJ
Autophagy 2020 Nov;16(11):2004-2016

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Histone deacetylase 3 is required for iNKT cell development.

Thapa P, Romero Arocha S, Chung JY, Sant'Angelo DB, Shapiro VS
Scientific reports 2017 Jul 19;7(1):5784

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Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia.

Marzetti E, Lorenzi M, Landi F, Picca A, Rosa F, Tanganelli F, Galli M, Doglietto GB, Pacelli F, Cesari M, Bernabei R, Calvani R, Bossola M
Experimental gerontology 2017 Jan;87(Pt A):92-99

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Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.

Bernard M, Dieudé M, Yang B, Hamelin K, Underwood K, Hébert MJ
Autophagy 2014;10(12):2193-207

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ATG7 by Simple WesternTM. Detection of Human ATG7 by Simple WesternTM.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ATG7 in Human Brain. ATG7 in Human Brain.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.