Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F46229 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ATG7 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ATG7 antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 antibody and 293 lysate transiently transfected with the human gene.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of ATG7 antibody and mouse liver tissue lysate. Predicted molecular weight: 70-80 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ATG7 antibody western blot analysis in HeLa lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the ATG7 antibody