Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00346 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ATG7 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for ATG7 detection. Tested with WB, IHC-P, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references LncRNA THRIL, transcriptionally activated by AP-1 and stabilized by METTL14-mediated m6A modification, accelerates LPS-evoked acute injury in alveolar epithelial cells.
Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension.
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis.
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma.
Shi Q, Li Z, Dong Y, Yang G, Li M
International immunopharmacology 2023 Oct;123:110740
International immunopharmacology 2023 Oct;123:110740
Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension.
Yi J, Wang X, Song K, Ren J, Che H, Yu H, Li Q
Journal of pharmaceutical and biomedical analysis 2022 Apr 1;212:114662
Journal of pharmaceutical and biomedical analysis 2022 Apr 1;212:114662
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis.
Zhong G, Long H, Ma S, Shunhan Y, Li J, Yao J
Life sciences 2019 Jun 1;226:164-172
Life sciences 2019 Jun 1;226:164-172
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma.
Zhang Y, Liu Y, Zou J, Yan L, Du W, Zhang Y, Sun H, Lu P, Geng S, Gu R, Zhang H, Bi Z
Oncotarget 2017 Oct 31;8(53):91134-91149
Oncotarget 2017 Oct 31;8(53):91134-91149
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 using anti-ATG7 antibody (A00346). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: rat spleen tissue lysates, Lane 9: mouse SP2/0 whole cell lystates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG7 antigen affinity purified polyclonal antibody (Catalog # A00346) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
- Additional image