PAB0742

antibody from Abnova Corporation

Targeting: ATG7 APG7L, DKFZp434N0735, GSA7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0742

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0742, RRID:AB_1112905

Product name

ATG7 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ATG7.

Storage

Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100 (ECL) .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase (MPO) appearing in green. Using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line.

Validation comment

Immunofluorescence