Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN1449608 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term), (AA 547-577) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 547~577 amino acids from the C-term of Human APG7L Genename: ATG7
- Description
- Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 547-577
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Ding ZB, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J
Cancer research 2008 Nov 15;68(22):9167-75
Cancer research 2008 Nov 15;68(22):9167-75
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Autophagy ATG7 (C-term) Cat.-No AP32176PU-N in 293 cell line lysates transiently transfected with the ATG7 gene (2 μg/Lane).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Autophagy ATG7 (C-term) Cat.-No AP32176PU-N in 293 cell line lysate (35ug/lane).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy ATG7 (C-term) Cat.-No AP32176PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.