TA501649

antibody from OriGene

Targeting: AK5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501649

Provider

OriGene

Proper citation

OriGene Cat#TA501649, RRID:AB_11128577

Product name

Anti-AK5 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Antibody type

Monoclonal

Description

Anti-AK5 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Host

Mouse

Conjugate

Unconjugated

Epitope

AK5

Isotype

IgG

Antibody clone number

OTI2D3

Vial size

100 µl

Concentration

0.47 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (RC222241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-AK5 mouse monoclonal antibody (TA501649) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5(RC222241).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC222241 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK5 antibody(TA501649), and then analyzed by flow cytometry.

Validation comment

FC