AP11577b

antibody from Abcepta

Targeting: TCF21 bHLHa23, POD1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP11577b

Provider

Abcepta

Proper citation

Abgent Cat#AP11577b, RRID:AB_10818611

Product name

TCF21 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody (C-term)(Cat. #AP11577b). AP11577b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TCF21 Antibody (C-term) (Cat. #AP11577b) western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the TCF21 antibody detected the TCF21 protein (arrow).

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TCF21 Antibody (C-term) (Cat. #AP11577b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50