Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-88637 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88637, RRID:AB_11008184
- Product name
- Rabbit Polyclonal TCF21 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human TCF21 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Characterization of TCF21 Downstream Target Regions Identifies a Transcriptional Network Linking Multiple Independent Coronary Artery Disease Loci.
Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap.
Sazonova O, Zhao Y, Nürnberg S, Miller C, Pjanic M, Castano VG, Kim JB, Salfati EL, Kundaje AB, Bejerano G, Assimes T, Yang X, Quertermous T
PLoS genetics 2015 May;11(5):e1005202
PLoS genetics 2015 May;11(5):e1005202
Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap.
Nurnberg ST, Cheng K, Raiesdana A, Kundu R, Miller CL, Kim JB, Arora K, Carcamo-Oribe I, Xiong Y, Tellakula N, Nanda V, Murthy N, Boisvert WA, Hedin U, Perisic L, Aldi S, Maegdefessel L, Pjanic M, Owens GK, Tallquist MD, Quertermous T
PLoS genetics 2015 May;11(5):e1005155
PLoS genetics 2015 May;11(5):e1005155
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF21 Antibody [NBP1-88637] - Analysis in control (vector only transfected HEK293T lysate) and tCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF21 Antibody [NBP1-88637] - Analysis in control (vector only transfected HEK293T lysate) and TCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human cerebral cortex shows no nuclear positivity in either neurons or glial cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human endometrium shows moderate nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human kidney shows moderate nuclear positivity in a subset of tubules as well as in some glomerular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human lung shows strong nuclear positivity in a subset of pneumocytes.