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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-52984 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DPEP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SLIGVEGGHS IDSSLGVLRA LYQLGMRYLT LTHSCNTPWA DNWLVDTGDS EPQSQGLSPF GQRVVKELNR LGVLIDLAHV SVATMKATLQ LSRAPVIFSH SSAYSVCASR RN Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 82%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
Guan Y, Liang X, Ma Z, Hu H, Liu H, Miao Z, Linkermann A, Hellwege JN, Voight BF, Susztak K
Nature communications 2021 Aug 23;12(1):5078
Nature communications 2021 Aug 23;12(1):5078
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DPEP1 in control (vector only transfected HEK293T lysate) and DPEP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DPEP1 Polyclonal Antibody (Product # PA5-52984).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DPEP1 in human cell line Hep G2 using a DPEP1 Polyclonal Antibody (Product # PA5-52984) shows localization to nucleus and cell junctions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human colon using DPEP1 Polyclonal Antibody (Product # PA5-52984).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human testis using DPEP1 Polyclonal Antibody (Product # PA5-52984).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human liver using DPEP1 Polyclonal Antibody (Product # PA5-52984).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human kidney tissue shows strong cytoplasmic and membranous positivity in cells in tubules. Samples were probed using a DPEP1 Polyclonal Antibody (Product # PA5-52984).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human kidney and lymph node tissues using DPEP1 Polyclonal Antibody (Product # PA5-52984). Corresponding DPEP1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human colon, kidney, liver and testis using DPEP1 Polyclonal Antibody (Product # PA5-52984) (A) shows similar protein distribution across tissues to an independent DPEP1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DPEP1 in human lymph node using DPEP1 Polyclonal Antibody (Product # PA5-52984) shows low expression as expected.
