Ab127341

antibody from Aladdin Scientific

Targeting: SGK1 SGK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab127341

Provider

Aladdin Scientific

Product name

SGK1 Antibody

Antibody type

Polyclonal

Description

Rabbit anti Human SGK1 Antibody, Polyclonal, could be used for WB, ICC, IF and so on.ApplicationWB: 1/1000ICC/IF: 1/25

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 70-103


Vial size

100_l,10_l,1ml,50_l

Concentration

0,5 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

SGK1 Antibody (Ab127341) - Western Blot All lanes: SGK1 Antibody (Ab127341) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 53 kDa Exposure time: 32.8 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

SGK1 Antibody (Ab127341) - IF/ICC Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SGK with SGK1 Antibody (Ab127341) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).