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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-54901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AFMID Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VSGVGFPSKV PWKKMSAEEL ENQYCPSRWV VRLGAEEALR TYSQIGIEAT TRARATRKSL LHVPYGD
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AFMID in control (vector only transfected HEK293T lysate) and AFMID over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a AFMID Polyclonal Antibody (Product # PA5-54901).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AFMID in human cell line Hep G2 using an AFMID Polyclonal Antibody (Product # PA5-54901) shows localization to mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AFMID in human cerebellum tissue shows strong cytoplasmic positivity in purkinje cells. Samples were probed using an AFMID Polyclonal Antibody (Product # PA5-54901).
