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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-88444 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GIPC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPLKLRGKKK AKSKETAGLV EGEPTGAGGG SLSASRAPAR RLVFHAQLAH GSATGRVEGF SSIQELYAQI AGAFEISPSE ILYCTLNTPK IDMERLLGGQ LGLEDFIFAH VKGIEKEVNV YKSEDSLGLT ITDNGVGYAF IKRIKDGGVI DSVKTICVGD HIESINGENI VGWRHYDVAK KLKELKKEEL FTMKLIEPKK AFEIELRSKA GKSSGEKIGC GRATLRLRSK GPATVEEMPS ETKAKAIEKI DDVLELYMGI RDIDLATTMF EAGKDKVNPD EFAVALDETL GDFAFPDEFV FDVWGVIGDA KRRGL; Positive Samples: U-251MG; Cellular Location: Cytoplasm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using GIPC2 Polyclonal antibody (Product # PA5-88444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GIPC2 in paraffin-embedded human colon using GIPC2 Polyclonal Antibody (Product # PA5-88444) at a dilution of 1:100.
