PA5-65370

antibody from Invitrogen Antibodies

Targeting: IFI44 MTAP44, p44, TLDC5

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65370

Provider

Invitrogen Antibodies

Product name

IFI44 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: CVAFVFDASSI QYFSSQMIVK IKRIRRELVN AGVVHVALLT HVDSMDLITK GDLIEIERCE PVRSKLEEVQ RKLGFALSDI SVVSNYSSEW ELD Highest antigen sequence identity to the following orthologs - mouse 60%, rat 59%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.15 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Interferon-Induced Protein 44 and Interferon-Induced Protein 44-Like Restrict Replication of Respiratory Syncytial Virus.

Busse DC, Habgood-Coote D, Clare S, Brandt C, Bassano I, Kaforou M, Herberg J, Levin M, Eléouët JF, Kellam P, Tregoning JS
Journal of virology 2020 Aug 31;94(18)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of IFI44 in human cell line HeLa shows localization to nucleoplasm. Samples were probed using an IFI44 Polyclonal Antibody (Product # PA5-65370).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIG 2 Overexpression of IFI44 or IFI44L reduces RSV infection. (a) IFI44 , IFI44L , and Mx1 mRNA levels relative to that of GAPDH in stably transduced overexpressing monoclonal A549 cell lines. Cells were cultured in normal growth medium or medium supplemented with 500 IU ml -1 IFN-alpha2a for 24 h. (b) Stably transduced cell lines were infected with rgRSV (MOI of 0.1 or 0.01) and infectivity assessed by flow cytometry after 24 h. Representative experiment showing GFP + population of single RFP + cells. (c) Comparison of PFU and GFP + IFU. (d) RSV infection of stably transduced cell lines relative to the vector control. N >= 5. (e) RSV L gene 24 h postinfection with wild-type RSV A2 relative to vector control. (f) Viral titer (WT RSV A2) relative to vector control in cell supernatant at 48 h postinfection by plaque assay. (g) A549 cells were transduced with either FLUC (vector) or IFI44 lentivirus, and the expression of IFI44 was detected by Western blotting after 48 h. (h) Transduced A549 cells were infected with rgRSV (MOI of 0.8) after 24 h, and the infectivity of transduced (RFP + ) cells was assessed 24 h after infection. N = 3. Individual points represent the results from an independent experiment. Bars show the means +- SEM. Asterisks represent significance relative to values for cells transduced with empty vector, assessed by ANOVA (a) or ratio-paired t test (c to e). Analysis was done prior to data transformation. * , P < 0.05; * * , P < 0.01; ** * , P < 0.001; *** *