Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47864 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CANT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-human Calcium Activated Nucleotidase 1/CANT1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47864) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CANT1 in LNCaP human prostate cancer cell line. Samples were incubated in CANT1 polyclonal antibody (Product # PA5-47864) using a dilution of 0.2 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CANT1 in immersion fixed PC‚3 human prostate cancer cell line. Samples were incubated in CANT1 polyclonal antibody (Product # PA5-47864) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (green) and counterstained with DAPI (blue). Specific staining was localized to perinuclei.