12442-2-AP

antibody from Invitrogen Antibodies

Targeting: PDE1A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12442-2-AP

Provider

Invitrogen Antibodies

Product name

PDE1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TRYDLINRF KIPVSCLITF AEALEVGYSK YKNPYHNLIH AADVTQTVHY IMLHTGIMHW LTELEILAMV FAAAIHDYEH TGTTNNFHIQ TRSDVAILYN DRSVLENHHV SAAYRLMQEE EMNILINLSK DDWRDLRNLV IEMVLSTDMS GHFQQIKNIR NSLQQPEGID RAKTMSLILH AADISHPAKS WKLHYRWTMA LMEEFFLQGD KEAELGLPFS PLCDRKSTMV AQSQIGFIDF IVEPTFSLLT DSTEKIVIPL IEEASKAETS SYVASSSTTI VGLHIADALR RSNTKGSMSD GSYSPDYSLA AVDLKSFKNN LVDIIQQNKE RWKELAAQGE SDLHKNSEDL VNAEEKHDET HS (185-545 aa encoded by BC022480)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 12442-2-AP) (PDE1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDE1A (IP:12442-2-AP, 4ug; Detection:12442-2-AP 1:500) with mouse brain tissue lysate 4000ug.