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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF5160 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse ENPP-7/Alk-SMase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse ENPP-7/Alk-SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human ENPP-7 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_001025462- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse ENPP-7/Alk-SMase by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse ENPP-7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP-7/Alk-SMase at approximately 60kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ENPP-7/Alk-SMase in intestine. ENPP-7/Alk-SMase was detected in perfusion fixed frozen sections of mouse intestine using Mouse ENPP-7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
