17504-1-AP

antibody from Proteintech Group

Targeting: EIF4A3 DDX48, EIF4AIII, Fal1, KIAA0111

Provider product page for 17504-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [69]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 17504-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17504-1-AP, RRID:AB_2097393
Product name: EIF4A3 antibody
Antibody type: Polyclonal
Description: KD/KO validated EIF4A3 antibody (Cat. #17504-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EIF4A3 protein structure - 17504-1-AP shown in red.

Submitted references Alu repeat-containing RNAs spatially organize actively transcribed genomic regions around nuclear speckles.

Liu S, Chen Z, Shen X, Gao J, Guo J, Rong Y, Li T, Zhou F, Si Y, Zhao H, Cheng T, Shi L, Yu J, Ma Y
Molecular cell 2026 May 7;86(9):1723-1741.e11

ZXDB Drives Macrophage Inflammatory Programming in Sepsis-Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA Translation.

Wu H, Gang W, Wang L, Sun J, Li S, Guo Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Mar 31;40(6):e71657

CircRSF1 Promoted HepG2 Cell Proliferation, Migration and Invasion Depending on EIF4A3.

Huang D, Zhu Z
Digestive diseases and sciences 2026 Mar 17;

EIF4A3/circPTGR1/miR-4725-5p positive- feedback loop promotes colorectal cancer progression via FAK/AKT signaling pathway.

Dong Y, Ding YH, Yang X, Song XH, Lei XT, Tang YN, Zhang XE, Huang WC, Zhang K, Chen JS
Molecular cancer 2026 Jan 5;25(1):28

Activation of central Angiotensin-(1-7)/Mas receptor alleviates synaptic damage in diabetes-associated cognitive impairment via modulating AKT/FOXO1/PACAP axis.

Tian S, Wu T, Zhang Z, Lv S, Ji X, Zhao Z, Ma X, Wang J, Bi Y
International journal of biological sciences 2025;21(6):2824-2842

A non-coding RNA circGNAI2 suppresses triple-negative breast cancer progression by sponging miR-454-3p to inhibit the VGLL4-STAT3 signaling.

Song H, Peng J, Niu Z, Liu X, Wang X, Wang Y, Lv M, Mao Y, Wang Y, Bhagat I, Wang H
The Journal of biological chemistry 2025 Sep;301(9):110577

Aerobic exercise alleviates chronic allergic airway inflammation by regulating the circMETTL9/EIF4A3/IGFBP3 axis.

Wang H, Jia Y, Ma B, Gao T, Wei H, Li D, Gu J, Chen O, Yue S
Cellular signalling 2025 Oct;134:111889

CircITSN1/EIF4A3/Itsn1 axis mediates postoperative cognitive dysfunction in aged mice: A novel mechanism and therapeutic target.

Zhang C, Zhu X, Gao R, Chen H, Yan C, Liu W, Yang L, Zeng X, Yang H, Liu J, Li Q, Ma D, Zhu T, Chen C
Molecular therapy. Nucleic acids 2025 Jun 10;36(2):102555

EIF4A3-Induced Circ_0059914 Promoted Angiogenesis and EMT of Glioma via the miR-1249/VEGFA Pathway.

Yu W, Chen D, Ma L, Lin Y, Zheng J, Li X
Molecular neurobiology 2025 Jan;62(1):973-987

SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay.

Sun Y, Zhang H, Shi DB, Gao P
Cell death & disease 2025 Jan 29;16(1):54

A novel peptide encoded by circSRCAP confers resistance to enzalutamide by inhibiting the ubiquitin-dependent degradation of AR-V7 in castration-resistant prostate cancer.

Meng X, Wu Q, Cao C, Yang W, Chu S, Guo H, Qi S, Bai J
Journal of translational medicine 2025 Jan 23;23(1):108

EIF4A3-Mediated circ_0008126 Inhibits the Progression and Metastasis of Gastric Cancer by Modulating the APC/β-Catenin Pathway.

Wang Z, Chen W, Wang Z, Dai X
Cancers 2025 Jan 14;17(2)

Aerobic exercise alleviates allergic airway inflammation by suppressing circMETTL9-mediated formation of macrophage extracellular traps.

Wang H, Gao T, Ma B, Jia Y, Wei H, Li D, Gu J, Chen O, Yue S
Non-coding RNA research 2025 Dec;15:156-171

CircZFR/YTHDF3 axis drives lymph node metastasis in cervical cancer via FASN translation.

Zhou M, Gao Y, Zhang Y, He L, Gao B, Zhang Y, Claret FX, Calin GA, Wang D
Molecular cancer 2025 Aug 21;24(1):218

Amplified dosage of the NKX2-1 lineage transcription factor controls its oncogenic role in lung adenocarcinoma.

Pulice JL, Meyerson M
Molecular cell 2025 Apr 3;85(7):1311-1329.e16

Circular RNA TAF4B targeting MFN2 accelerates cell growth in bladder cancer through p27 depression and AKT activation.

Zhang X, Xu J, Zhuang G, Wang Y, Li X, Zhu X
Frontiers in immunology 2024;15:1477196

CircHIPK2 Contributes Cell Growth in Intestinal Epithelial of Colitis and Colorectal Cancer through Promoting TAZ Translation.

Zeng X, Tang J, Zhang Q, Wang C, Qi J, Wei Y, Xu J, Yang K, Zhou Z, Wu H, Luo J, Jiang Y, Song Z, Wu J, Wu J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Sep;11(34):e2401588

circE2F1-encoded peptide inhibits circadian machinery essential for nucleotide biosynthesis and tumor progression via repressing SPIB/E2F1 axis.

Wang J, Wang X, Yang C, Li Q, Li D, Du X, Cheng Y, Tian M, Zheng L, Tong Q
International journal of biological macromolecules 2024 Sep 15;280(Pt 1):135698

Nucleolar dynamics are determined by the ordered assembly of the ribosome.

Sheu-Gruttadauria J, Yan X, Stuurman N, Vale RD, Floor SN
bioRxiv : the preprint server for biology 2024 Oct 15;

Radiation-sensitive circRNA hsa_circ_0096498 inhibits radiation-induced liver fibrosis by suppressing EIF4A3 nuclear translocation to decrease CDC42 expression in hepatic stellate cells.

Zhou P, Deng Y, Sun Y, Wu D, Chen Y
Journal of translational medicine 2024 Oct 1;22(1):884

The oncogenic role of EIF4A3/CDC20 axis in the endometrial cancer.

Lin Y, Kong L, Zhao Y, Zhai F, Zhan Z, Li Y, Jingfei Z, Chunhong Y, Jin X
Journal of molecular medicine (Berlin, Germany) 2024 Nov;102(11):1395-1410

Human disease-causing mutations result in loss of leiomodin 2 through nonsense-mediated mRNA decay.

Pappas CT, Mayfield RM, Dickerson AE, Mi-Mi L, Gregorio CC
PLoS genetics 2024 May;20(5):e1011279

Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle.

Choi Y, Um B, Na Y, Kim J, Kim JS, Kim VN
Molecular cell 2024 May 2;84(9):1764-1782.e10

A unique circ_0067716/EIF4A3 double-negative feedback loop impacts malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene.

Zhao S, Xiao M, Li L, Zhang H, Shan M, Cui S, Zhang L, Zhang G, Wu S, Jin C, Yang J, Lu X
The Science of the total environment 2024 May 1;923:171349

CircGLIS3 promotes gastric cancer progression by regulating the miR-1343-3p/PGK1 pathway and inhibiting vimentin phosphorylation.

Zhang Y, Wang X, Liu W, Lei T, Qiao T, Feng W, Song W
Journal of translational medicine 2024 Mar 8;22(1):251

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression.

Li L, Liu D, Chen T, Wei C, Qiao Y, Liu W, Liang Y, Liang Z, Chen C, Li D, Wu B, Zhao X, Huang D, Wu D
Journal of experimental & clinical cancer research : CR 2024 Jul 20;43(1):200

Integrating clinical data mining, network analysis and experimental validation reveal the anti-inflammatory mechanism of Huangqin Qingre Chubi Capsule in rheumatoid arthritis treatment.

Hu Y, Liu J, Qi Y, Zhou Q, Li Y, Cong C, Chen Y
Journal of ethnopharmacology 2024 Jul 15;329:118077

EIF4A3-mediated biogenesis of circSTX6 promotes bladder cancer metastasis and cisplatin resistance.

Wei W, Liu K, Huang X, Tian S, Wang H, Zhang C, Ye J, Dong Y, An Z, Ma X, Wang B, Huang Y, Zhang X
Journal of experimental & clinical cancer research : CR 2024 Jan 2;43(1):2

M6A reader YTHDF1 promotes malignant progression of laryngeal squamous carcinoma through activating the EMT pathway by EIF4A3.

Guo H, Han Q, Guan X, Li Z, Wang Y, He L, Guo Y, Zhao L, Xue X, Liu H, Zhang C
Cellular signalling 2024 Feb;114:111002

Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3.

Yuan LX, Luo M, Liu RY, Wang HX, Ju LL, Wang F, Cao YL, Wang ZC, Chen L
BMC cancer 2024 Feb 21;24(1):239

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation.

Xue M, Dong L, Zhang H, Li Y, Qiu K, Zhao Z, Gao M, Han L, Chan AKN, Li W, Leung K, Wang K, Pokharel SP, Qing Y, Liu W, Wang X, Ren L, Bi H, Yang L, Shen C, Chen Z, Melstrom L, Li H, Timchenko N, Deng X, Huang W, Rosen ST, Tian J, Xu L, Diao J, Chen CW, Chen J, Shen B, Chen H, Su R
Journal of hematology & oncology 2024 Feb 1;17(1):7

Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.

Hu Y, Cai ZR, Huang RZ, Wang DS, Ju HQ, Chen DL
Molecular cancer 2024 Dec 18;23(1):272

Identification of m6A suppressor EIF4A3 as a novel cancer prognostic and immunotherapy biomarker through bladder cancer clinical data validation and pan-cancer analysis.

Yan H, Zhang L, Li R
Scientific reports 2023 Sep 30;13(1):16457

Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma.

Pulice JL, Meyerson M
bioRxiv : the preprint server for biology 2023 Oct 27;

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Curdy N, Lanvin O, Cerapio JP, Pont F, Tosolini M, Sarot E, Valle C, Saint-Laurent N, Lhuillier E, Laurent C, Fournié JJ, Franchini DM
Cell reports 2023 Mar 28;42(3):112211

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway.

Yang X, Han F, Hu X, Li G, Wu H, Can C, Wei Y, Liu J, Wang R, Jia W, Ji C, Ma D
Biomarker research 2023 Jun 6;11(1):59

EIF4A3-Induced Upregulation of hsa_circ_0040039 is a Biomarker and Aggravates IL-1β-Stimulated Intervertebral Disc Degeneration.

Li L, Cao Y, Deng H, Jia J, Fan X
Applied biochemistry and biotechnology 2023 Jul;195(7):4292-4307

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling.

Liu X, Guo Q, Gao G, Cao Z, Guan Z, Jia B, Wang W, Zhang K, Zhang W, Wang S, Li W, Hao Q, Zhang Y, Li M, Zhang W, Gu J
Journal of nanobiotechnology 2023 Feb 8;21(1):45

CircKIF4A combines EIF4A3 to stabilize SDC1 expression to activate c-src/FAK and promotes TNBC progression.

Luo P, Gong Y, Weng J, Wen F, Luo J, Hu C, Xiao J, Shu J
Cellular signalling 2023 Aug;108:110690

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Chen T, Yang H, Liu P, Hamiti M, Zhang X, Xu Y, Quan W, Zhang Y, Yu W, Jiao L, Du T, Xi J, Yin B, Zhou W, Lu S, Peng X
Virologica Sinica 2023 Apr;38(2):222-232

Epitranscriptic regulation of HRAS by N(6)-methyladenosine drives tumor progression.

Pan Y, Gu Y, Liu T, Zhang Q, Yang F, Duan L, Cheng S, Zhu X, Xi Y, Chang X, Ye Q, Gao S
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 4;120(14):e2302291120

CircMYH9 increases KPNA2 mRNA stability to promote hepatocellular carcinoma progression in an EIF4A3-dependent manner.

Xia J, Wu C, Tang Y, Tang J, Zhu D, Zhang F, Xu Z, Sun D, Tan Z, Zhuo H
American journal of cancer research 2022;12(9):4361-4372

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J
Nucleic acids research 2022 Nov 28;50(21):12462-12479

eIF4A3 Promotes RNA Viruses' Replication by Inhibiting Innate Immune Responses.

Gao Q, Jiang M, Zhao Y, Li G, Yang C, Ren C, Jin M, Chen H, Zhou H
Journal of virology 2022 Nov 23;96(22):e0151322

EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.

Wang X, Song H, Fang L, Wu T
Cell death discovery 2022 Mar 2;8(1):92

The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells.

Nagel M, Noß M, Xu J, Horn N, Ueffing M, Boldt K, Schüle R
RNA (New York, N.Y.) 2022 Dec 16;29(1):55-68

Selenium Restores Synaptic Deficits by Modulating NMDA Receptors and Selenoprotein K in an Alzheimer's Disease Model.

Zhang ZH, Chen C, Jia SZ, Cao XC, Liu M, Tian J, Hoffmann PR, Xu HX, Ni JZ, Song GL
Antioxidants & redox signaling 2021 Oct 10;35(11):863-884

CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs.

Wang X, Jin P, Zhang Y, Wang K
Cell death & disease 2021 Mar 19;12(4):297

Local translation in nuclear condensate amyloid bodies.

Theodoridis PR, Bokros M, Marijan D, Balukoff NC, Wang D, Kirk CC, Budine TD, Goldsmith HD, Wang M, Audas TE, Lee S
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Sakellariou D, Tiberti M, Kleiber TH, Blazquez L, López AR, Abildgaard MH, Lubas M, Bartek J, Papaleo E, Frankel LB
Cell death and differentiation 2021 Dec;28(12):3344-3356

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Park MK, Zhang L, Min KW, Cho JH, Yeh CC, Moon H, Hormaechea-Agulla D, Mun H, Ko S, Lee JW, Jathar S, Smith AS, Yao Y, Giang NT, Vu HH, Yan VC, Bridges MC, Kourtidis A, Muller F, Chang JH, Song SJ, Nakagawa S, Hirose T, Yoon JH, Song MS
Cell metabolism 2021 Dec 7;33(12):2380-2397.e9

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation.

Feng J, Ren X, Fu H, Li D, Chen X, Zu X, Liu Q, Wu M
Molecular therapy. Nucleic acids 2021 Dec 3;26:473-487

hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3.

Wang X, Chen M, Fang L
Molecular therapy. Nucleic acids 2021 Dec 3;26:122-134

Comprehensive analysis of biological networks and the eukaryotic initiation factor 4A-3 gene as pivotal in hepatocellular carcinoma.

Lin Y, Liang R, Mao Y, Ye J, Mai R, Gao X, Liu Z, Wainwright T, Li Q, Luo M, Ge L, Li Y, Zou D
Journal of cellular biochemistry 2020 Oct;121(10):4094-4107

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.

Wu H, Qin W, Lu S, Wang X, Zhang J, Sun T, Hu X, Li Y, Chen Q, Wang Y, Zhao H, Piao H, Zhang R, Wei M
Molecular cancer 2020 May 22;19(1):95

LINC00680 and TTN-AS1 Stabilized by EIF4A3 Promoted Malignant Biological Behaviors of Glioblastoma Cells.

Tang W, Wang D, Shao L, Liu X, Zheng J, Xue Y, Ruan X, Yang C, Liu L, Ma J, Li Z, Liu Y
Molecular therapy. Nucleic acids 2020 Mar 6;19:905-921

LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.

Qi W, Li Z, Xia L, Dai J, Zhang Q, Wu C, Xu S
Scientific reports 2019 Nov 7;9(1):16185

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.

Jiao AL, Perales R, Umbreit NT, Haswell JR, Piper ME, Adams BD, Pellman D, Kennedy S, Slack FJ
RNA (New York, N.Y.) 2019 Mar;25(3):352-363

LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.

Luo K, Geng J, Zhang Q, Xu Y, Zhou X, Huang Z, Shi KQ, Pan C, Wu J
Journal of experimental & clinical cancer research : CR 2019 Jun 11;38(1):249

mRNAs containing NMD-competent premature termination codons are stabilized and translated under UPF1 depletion.

Kim WK, Yun S, Kwon Y, You KT, Shin N, Kim J, Kim H
Scientific reports 2017 Nov 20;7(1):15833

YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.

Shi H, Wang X, Lu Z, Zhao BS, Ma H, Hsu PJ, Liu C, He C
Cell research 2017 Mar;27(3):315-328

Y14 governs p53 expression and modulates DNA damage sensitivity.

Lu CC, Lee CC, Tseng CT, Tarn WY
Scientific reports 2017 Mar 31;7:45558

The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation.

Rodor J, Pan Q, Blencowe BJ, Eyras E, Cáceres JF
RNA (New York, N.Y.) 2016 Sep;22(9):1411-26

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Prpar Mihevc S, Baralle M, Buratti E, Rogelj B
Scientific reports 2016 Sep 26;6:33996

A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.

Chuang TW, Lee KM, Lou YC, Lu CC, Tarn WY
The Journal of biological chemistry 2016 Apr 15;291(16):8565-74

A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups.

Jang MK, Anderson DE, van Doorslaer K, McBride AA
Proteomics 2015 Jun;15(12):2038-50

A fine balance: Regulation of hippocampal Arc/Arg3.1 transcription, translation and degradation in a rat model of normal cognitive aging.

Fletcher BR, Hill GS, Long JM, Gallagher M, Shapiro ML, Rapp PR
Neurobiology of learning and memory 2014 Nov;115:58-67

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.

Melero R, Uchiyama A, Castaño R, Kataoka N, Kurosawa H, Ohno S, Yamashita A, Llorca O
Structure (London, England : 1993) 2014 Aug 5;22(8):1105-1119

An atlas of chromatoid body components.

Meikar O, Vagin VV, Chalmel F, Sõstar K, Lardenois A, Hammell M, Jin Y, Da Ros M, Wasik KA, Toppari J, Hannon GJ, Kotaja N
RNA (New York, N.Y.) 2014 Apr;20(4):483-95

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP(EIF4A3 antibody) at dilution of 1:600
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 17504-1-AP(EIF4A3 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue