17504-1-AP

antibody from Proteintech Group

Targeting: EIF4A3 DDX48, EIF4AIII, Fal1, KIAA0111

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17504-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17504-1-AP, RRID:AB_2097393

Product name

EIF4A3 antibody

Antibody type

Polyclonal

Description

KD/KO validated EIF4A3 antibody (Cat. #17504-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Nucleolar dynamics are determined by the ordered assembly of the ribosome.

Sheu-Gruttadauria J, Yan X, Stuurman N, Vale RD, Floor SN
bioRxiv : the preprint server for biology 2024 Oct 15;

---

Human disease-causing mutations result in loss of leiomodin 2 through nonsense-mediated mRNA decay.

Pappas CT, Mayfield RM, Dickerson AE, Mi-Mi L, Gregorio CC
PLoS genetics 2024 May;20(5):e1011279

---

Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle.

Choi Y, Um B, Na Y, Kim J, Kim JS, Kim VN
Molecular cell 2024 May 2;84(9):1764-1782.e10

---

A unique circ_0067716/EIF4A3 double-negative feedback loop impacts malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene.

Zhao S, Xiao M, Li L, Zhang H, Shan M, Cui S, Zhang L, Zhang G, Wu S, Jin C, Yang J, Lu X
The Science of the total environment 2024 May 1;923:171349

---

CircGLIS3 promotes gastric cancer progression by regulating the miR-1343-3p/PGK1 pathway and inhibiting vimentin phosphorylation.

Zhang Y, Wang X, Liu W, Lei T, Qiao T, Feng W, Song W
Journal of translational medicine 2024 Mar 8;22(1):251

---

Integrating clinical data mining, network analysis and experimental validation reveal the anti-inflammatory mechanism of Huangqin Qingre Chubi Capsule in rheumatoid arthritis treatment.

Hu Y, Liu J, Qi Y, Zhou Q, Li Y, Cong C, Chen Y
Journal of ethnopharmacology 2024 Jul 15;329:118077

---

EIF4A3-mediated biogenesis of circSTX6 promotes bladder cancer metastasis and cisplatin resistance.

Wei W, Liu K, Huang X, Tian S, Wang H, Zhang C, Ye J, Dong Y, An Z, Ma X, Wang B, Huang Y, Zhang X
Journal of experimental & clinical cancer research : CR 2024 Jan 2;43(1):2

---

M6A reader YTHDF1 promotes malignant progression of laryngeal squamous carcinoma through activating the EMT pathway by EIF4A3.

Guo H, Han Q, Guan X, Li Z, Wang Y, He L, Guo Y, Zhao L, Xue X, Liu H, Zhang C
Cellular signalling 2024 Feb;114:111002

---

Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3.

Yuan LX, Luo M, Liu RY, Wang HX, Ju LL, Wang F, Cao YL, Wang ZC, Chen L
BMC cancer 2024 Feb 21;24(1):239

---

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation.

Xue M, Dong L, Zhang H, Li Y, Qiu K, Zhao Z, Gao M, Han L, Chan AKN, Li W, Leung K, Wang K, Pokharel SP, Qing Y, Liu W, Wang X, Ren L, Bi H, Yang L, Shen C, Chen Z, Melstrom L, Li H, Timchenko N, Deng X, Huang W, Rosen ST, Tian J, Xu L, Diao J, Chen CW, Chen J, Shen B, Chen H, Su R
Journal of hematology & oncology 2024 Feb 1;17(1):7

---

Identification of m6A suppressor EIF4A3 as a novel cancer prognostic and immunotherapy biomarker through bladder cancer clinical data validation and pan-cancer analysis.

Yan H, Zhang L, Li R
Scientific reports 2023 Sep 30;13(1):16457

---

Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma.

Pulice JL, Meyerson M
bioRxiv : the preprint server for biology 2023 Oct 27;

---

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Curdy N, Lanvin O, Cerapio JP, Pont F, Tosolini M, Sarot E, Valle C, Saint-Laurent N, Lhuillier E, Laurent C, Fournié JJ, Franchini DM
Cell reports 2023 Mar 28;42(3):112211

---

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway.

Yang X, Han F, Hu X, Li G, Wu H, Can C, Wei Y, Liu J, Wang R, Jia W, Ji C, Ma D
Biomarker research 2023 Jun 6;11(1):59

---

EIF4A3-Induced Upregulation of hsa_circ_0040039 is a Biomarker and Aggravates IL-1β-Stimulated Intervertebral Disc Degeneration.

Li L, Cao Y, Deng H, Jia J, Fan X
Applied biochemistry and biotechnology 2023 Jul;195(7):4292-4307

---

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling.

Liu X, Guo Q, Gao G, Cao Z, Guan Z, Jia B, Wang W, Zhang K, Zhang W, Wang S, Li W, Hao Q, Zhang Y, Li M, Zhang W, Gu J
Journal of nanobiotechnology 2023 Feb 8;21(1):45

---

CircKIF4A combines EIF4A3 to stabilize SDC1 expression to activate c-src/FAK and promotes TNBC progression.

Luo P, Gong Y, Weng J, Wen F, Luo J, Hu C, Xiao J, Shu J
Cellular signalling 2023 Aug;108:110690

---

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Chen T, Yang H, Liu P, Hamiti M, Zhang X, Xu Y, Quan W, Zhang Y, Yu W, Jiao L, Du T, Xi J, Yin B, Zhou W, Lu S, Peng X
Virologica Sinica 2023 Apr;38(2):222-232

---

Epitranscriptic regulation of HRAS by N(6)-methyladenosine drives tumor progression.

Pan Y, Gu Y, Liu T, Zhang Q, Yang F, Duan L, Cheng S, Zhu X, Xi Y, Chang X, Ye Q, Gao S
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 4;120(14):e2302291120

---

CircMYH9 increases KPNA2 mRNA stability to promote hepatocellular carcinoma progression in an EIF4A3-dependent manner.

Xia J, Wu C, Tang Y, Tang J, Zhu D, Zhang F, Xu Z, Sun D, Tan Z, Zhuo H
American journal of cancer research 2022;12(9):4361-4372

---

The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells.

Nagel M, Noss M, Xu J, Horn N, Ueffing M, Boldt K, Schuele R
RNA (New York, N.Y.) 2022 Oct 31;29(1):55-68

---

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J
Nucleic acids research 2022 Nov 28;50(21):12462-12479

---

eIF4A3 Promotes RNA Viruses' Replication by Inhibiting Innate Immune Responses.

Gao Q, Jiang M, Zhao Y, Li G, Yang C, Ren C, Jin M, Chen H, Zhou H
Journal of virology 2022 Nov 23;96(22):e0151322

---

EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.

Wang X, Song H, Fang L, Wu T
Cell death discovery 2022 Mar 2;8(1):92

---

CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs.

Wang X, Jin P, Zhang Y, Wang K
Cell death & disease 2021 Mar 19;12(4):297

---

Local translation in nuclear condensate amyloid bodies.

Theodoridis PR, Bokros M, Marijan D, Balukoff NC, Wang D, Kirk CC, Budine TD, Goldsmith HD, Wang M, Audas TE, Lee S
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)

---

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Sakellariou D, Tiberti M, Kleiber TH, Blazquez L, López AR, Abildgaard MH, Lubas M, Bartek J, Papaleo E, Frankel LB
Cell death and differentiation 2021 Dec;28(12):3344-3356

---

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Park MK, Zhang L, Min KW, Cho JH, Yeh CC, Moon H, Hormaechea-Agulla D, Mun H, Ko S, Lee JW, Jathar S, Smith AS, Yao Y, Giang NT, Vu HH, Yan VC, Bridges MC, Kourtidis A, Muller F, Chang JH, Song SJ, Nakagawa S, Hirose T, Yoon JH, Song MS
Cell metabolism 2021 Dec 7;33(12):2380-2397.e9

---

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation.

Feng J, Ren X, Fu H, Li D, Chen X, Zu X, Liu Q, Wu M
Molecular therapy. Nucleic acids 2021 Dec 3;26:473-487

---

hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3.

Wang X, Chen M, Fang L
Molecular therapy. Nucleic acids 2021 Dec 3;26:122-134

---

Comprehensive analysis of biological networks and the eukaryotic initiation factor 4A-3 gene as pivotal in hepatocellular carcinoma.

Lin Y, Liang R, Mao Y, Ye J, Mai R, Gao X, Liu Z, Wainwright T, Li Q, Luo M, Ge L, Li Y, Zou D
Journal of cellular biochemistry 2020 Oct;121(10):4094-4107

---

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.

Wu H, Qin W, Lu S, Wang X, Zhang J, Sun T, Hu X, Li Y, Chen Q, Wang Y, Zhao H, Piao H, Zhang R, Wei M
Molecular cancer 2020 May 22;19(1):95

---

LINC00680 and TTN-AS1 Stabilized by EIF4A3 Promoted Malignant Biological Behaviors of Glioblastoma Cells.

Tang W, Wang D, Shao L, Liu X, Zheng J, Xue Y, Ruan X, Yang C, Liu L, Ma J, Li Z, Liu Y
Molecular therapy. Nucleic acids 2020 Mar 6;19:905-921

---

LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.

Qi W, Li Z, Xia L, Dai J, Zhang Q, Wu C, Xu S
Scientific reports 2019 Nov 7;9(1):16185

---

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.

Jiao AL, Perales R, Umbreit NT, Haswell JR, Piper ME, Adams BD, Pellman D, Kennedy S, Slack FJ
RNA (New York, N.Y.) 2019 Mar;25(3):352-363

---

LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.

Luo K, Geng J, Zhang Q, Xu Y, Zhou X, Huang Z, Shi KQ, Pan C, Wu J
Journal of experimental & clinical cancer research : CR 2019 Jun 11;38(1):249

---

mRNAs containing NMD-competent premature termination codons are stabilized and translated under UPF1 depletion.

Kim WK, Yun S, Kwon Y, You KT, Shin N, Kim J, Kim H
Scientific reports 2017 Nov 20;7(1):15833

---

YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.

Shi H, Wang X, Lu Z, Zhao BS, Ma H, Hsu PJ, Liu C, He C
Cell research 2017 Mar;27(3):315-328

---

Y14 governs p53 expression and modulates DNA damage sensitivity.

Lu CC, Lee CC, Tseng CT, Tarn WY
Scientific reports 2017 Mar 31;7:45558

---

The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation.

Rodor J, Pan Q, Blencowe BJ, Eyras E, Cáceres JF
RNA (New York, N.Y.) 2016 Sep;22(9):1411-26

---

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Prpar Mihevc S, Baralle M, Buratti E, Rogelj B
Scientific reports 2016 Sep 26;6:33996

---

A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.

Chuang TW, Lee KM, Lou YC, Lu CC, Tarn WY
The Journal of biological chemistry 2016 Apr 15;291(16):8565-74

---

A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups.

Jang MK, Anderson DE, van Doorslaer K, McBride AA
Proteomics 2015 Jun;15(12):2038-50

---

A fine balance: Regulation of hippocampal Arc/Arg3.1 transcription, translation and degradation in a rat model of normal cognitive aging.

Fletcher BR, Hill GS, Long JM, Gallagher M, Shapiro ML, Rapp PR
Neurobiology of learning and memory 2014 Nov;115:58-67

---

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.

Melero R, Uchiyama A, Castaño R, Kataoka N, Kurosawa H, Ohno S, Yamashita A, Llorca O
Structure (London, England : 1993) 2014 Aug 5;22(8):1105-1119

---

An atlas of chromatoid body components.

Meikar O, Vagin VV, Chalmel F, Sõstar K, Lardenois A, Hammell M, Jin Y, Da Ros M, Wasik KA, Toppari J, Hannon GJ, Kotaja N
RNA (New York, N.Y.) 2014 Apr;20(4):483-95

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP(EIF4A3 antibody) at dilution of 1:600

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 17504-1-AP(EIF4A3 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue