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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-30329 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-eIF4A3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-30329 targets EIF4A3 in IF and WB applications and shows reactivity with Human samples. The PA5-30329 immunogen is recombinant fragment corresponding to a region within amino acids 176 and 411 of Human EIF4A3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.28 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications 2015 Sep 18;6:8192
Nature communications 2015 Sep 18;6:8192
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4A3 using 30 µg of Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EIF4A3 polyclonal antibody (Product # PA5-30329) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF4A3 in paraformaldehyde-fixed A431 cells using an EIF4A3 polyclonal antibody (Product # PA5-30329) at a 1:500 dilution.
