PA5-120462

antibody from Invitrogen Antibodies

Targeting: CARD14 BIMP2, CARMA2, PSORS2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-120462

Provider

Invitrogen Antibodies

Product name

CARD14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HT-29, HeLa. The target is usually found in the following locations: Cytoplasm, Cytoplasm. Immunogen sequence: SLSLHYSNAL QEKELAASRC RSLQEELYLL KQELQRANMV SSCELELQEQ SLRTASDQES GDEELNRLKE ENEKLRSLTF SLAEKDILEQ SLDEARGSRQ ELVERIHSLR ERAVAAERQR EQYWEEKEQT LLQFQKSKMA CQLYREKVNA LQAQVCELQK ERDQAYSARD SAQREISQSL VEKDSLRRQV FELTDQVCEL RTQLRQLQAE PPGVLKQEAR TREPCPREKQ RLVRMHAICP RDDSDCSLVS STESQLLSDL SATSSRELVD SFRSSSPAPP SQQSLYKRVA EDFGEEPWSF S

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.12 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CARD14 in extracts of various cell lines. Samples were incubated with CARD14 Polyclonal antibody (Product # PA5-120462) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.