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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP22065PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22065PU-N, RRID:AB_10758330
- Product name
- anti NUP35 (50-100)
- Antibody type
- Polyclonal
- Antigen
- Amino acids 50-100 of human NUP35
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Zebrafish
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using AP22065PU-N at 1.0 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUP35 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and AP22065PU-N (bottom left, right) at 5 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human skin (epidermis) stained with NUP35 antibody (Cat.-No.: AP22065PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
