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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP22066PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22066PU-N, RRID:AB_10758476
- Product name
- anti NUP35 (280-330)
- Antibody type
- Polyclonal
- Antigen
- Amino acids 280-330 of human NUP35
- Reactivity
- Human, Mouse, Bovine, Canine, Chicken/Avian, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using AP22066PU-N at 2 µg/ml, 2 µg/ml, 1 µg/ml and 1 µg/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUP35 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and AP22066PU-N (bottom left, right) at 5 µg/ml.
