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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-21490 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL22RA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLaS3.
- Concentration
- 0.66 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IL22RA2 Polyclonal Antibody (Product # PA5-21490). Sample (30 µg of whole cell lysate). A: HeLa S3. 12% SDS PAGE. IL22RA2 Polyclonal Antibody (Product # PA5-21490) diluted at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL22RA2 Polyclonal Antibody detects IL22RA2 protein at cytosol on PC14 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC14 xenograft. IL22RA2 Polyclonal Antibody (Product # PA5-21490) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL22BP direct ELISA: 96 well were coated overnight with supernatant of HEK293 cells transfected with either adenovirus-mIL22BP (+C, with dilutions as indicated) or adenovirus control (-C) followed by blocking with 5% milk for 2 hour. Incubation with IL22BP polyclonal antibody (Product # PA5-21490) for 2 hours (5 µg/mL), followed by anti rabbit IgG- HRP (1:2000) for 30 minutes at room temperature. TMB until color development and stopped with sulfuric acid 2N. Data courtesy of Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA of IL22BP. Anti rabbit IgG- Capture anti IL22Ra2 were coated at the concentration of 100 ng/well overnight at 4C. Blocked with milk 5% for 2h. Added supernatant of HEK293 cells transfected with either adenovirus-mIL22BP or adenovirus control for 2 hours. Incubation with IL22BP polyclonal antibody (Product # PA5-21490) for 2 hours (5 µg/mL), followed by anti rabbit IgG- HRP (1:2000) for 30 minutes at room temperature. TMB until color development and stopped with sulfuric acid 2N. Data courtesy of Antibody Data Exchange Program.
