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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ACC-082-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPML2 (Mucolipin-2) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates: - 1-3. Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), (1:200).4-6. Anti-TRPML2 (Mucolipin-2) Antibody , preincubated with TRPML2/Mucolipin-2 Blocking Peptide (#BLP-CC082).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates: - 1-3. Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), (1:200).4-6. Anti-TRPML2 (Mucolipin-2) Antibody , preincubated with TRPML2/Mucolipin-2 Blocking Peptide (#BLP-CC082).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPML2 in mouse muscle myoblast (C2C12) cell line - Immunocytochemical staining of TRPML2 in C2C12 cells. A. Cells were stained with Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), (red), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. DAPI is used as the counter staining (blue). C. Merged images of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPML2 in rat heart - Immunohistochemical staining of rat heart paraffin-embedded sections using Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), followed by AlexaFluor-555 secondary Antibody . A. TRPML2 labeling appears specifically in the cardiac muscle fibers and in a lesser intensity in the endothelium. It does not appear in the smooth muscle fibers of blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
