AB5995

OLIG3 antibody from EMD Millipore
Bhlhb7, bHLHe20
Western blot
Recommended by provider
Immunohistochemistry
Recommended by provider

Antibody data

Product number
AB5995
Provider
EMD Millipore
Proper citation
Millipore Cat#AB5995, RRID:AB_2157690
Product name
Anti-Olig3 Antibody
Provider product page
EMD Millipore - AB5995
Antibody type
Polyclonal
Antigen
Synthetic peptide, amino acids 53-66 of Olig3.
Description
Affinity Purified
Reactivity
Human, Mouse
Host
Rabbit
Vial size
100 µg
Storage
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 µL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 µg of precipitated antibody, the amount of buffer needed would be 50 µL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Provider Type Product Number
- No reagents -