Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- NBP1-76741 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76741, RRID:AB_11036124
- Product name
- Rabbit Polyclonal PERP Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PERP Antibody [NBP1-76741] - Analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with using this antibody at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.