PA5-19980

antibody from Invitrogen Antibodies

Targeting: PERP dJ496H19.1, KCP1, KRTCAP1, PIGPC1, THW

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19980

Provider

Invitrogen Antibodies

Product name

PERP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~21kDa. A suggested positive control is A431 cell lysate. PA5-19980 can be used with blocking peptide PEP-0104.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide using a PERP polyclonal antibody (Product # PA5-19980) at 1 µg /mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 µg of lysates per lane. Antibodies: PERP Polyclonal Antibody (Product # PA5-19980) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 µg of lysates per lane. Antibodies: PERP Polyclonal Antibody (Product # PA5-19980) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PERP Polyclonal Antibody (Product # PA5-19980) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of A431 cells using PERP Polyclonal Antibody (Product # PA5-19980) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.