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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NB100-356 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-356, RRID:AB_10000986
- Product name
- Rabbit Polyclonal Cyr61/CCN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references YAP regulates cell size and growth dynamics via non-cell autonomous mediators.
Inhibition of CYR61-S100A4 Axis Limits Breast Cancer Invasion.
CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity.
Progesterone Alleviates Endometriosis via Inhibition of Uterine Cell Proliferation, Inflammation and Angiogenesis in an Immunocompetent Mouse Model.
Epidermal growth factor upregulates endometrial CYR61 expression via activation of the JAK2/STAT3 pathway.
Extracellular matrix protein CCN1 limits oncolytic efficacy in glioma.
Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells.
Silencing cAMP-response element-binding protein (CREB) identifies CYR61 as a tumor suppressor gene in melanoma.
Roles of ezrin in the growth and invasiveness of esophageal squamous carcinoma cells.
Premenstrual regulation of the pro-angiogenic factor CYR61 in human endometrium.
Mugahid D, Kalocsay M, Liu X, Gruver JS, Peshkin L, Kirschner MW
eLife 2020 Jan 9;9
eLife 2020 Jan 9;9
Inhibition of CYR61-S100A4 Axis Limits Breast Cancer Invasion.
Hellinger JW, Hüchel S, Goetz L, Bauerschmitz G, Emons G, Gründker C
Frontiers in oncology 2019;9:1074
Frontiers in oncology 2019;9:1074
CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity.
Park MH, Kim AK, Manandhar S, Oh SY, Jang GH, Kang L, Lee DW, Hyeon DY, Lee SH, Lee HE, Huh TL, Suh SH, Hwang D, Byun K, Park HC, Lee YM
eLife 2019 Aug 20;8
eLife 2019 Aug 20;8
Progesterone Alleviates Endometriosis via Inhibition of Uterine Cell Proliferation, Inflammation and Angiogenesis in an Immunocompetent Mouse Model.
Li Y, Adur MK, Kannan A, Davila J, Zhao Y, Nowak RA, Bagchi MK, Bagchi IC, Li Q
PloS one 2016;11(10):e0165347
PloS one 2016;11(10):e0165347
Epidermal growth factor upregulates endometrial CYR61 expression via activation of the JAK2/STAT3 pathway.
Klein R, Stiller S, Gashaw I
Reproduction, fertility, and development 2012;24(3):482-9
Reproduction, fertility, and development 2012;24(3):482-9
Extracellular matrix protein CCN1 limits oncolytic efficacy in glioma.
Haseley A, Boone S, Wojton J, Yu L, Yoo JY, Yu J, Kurozumi K, Glorioso JC, Caligiuri MA, Kaur B
Cancer research 2012 Mar 15;72(6):1353-62
Cancer research 2012 Mar 15;72(6):1353-62
Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells.
Xie JJ, Xu LY, Wu JY, Shen ZY, Zhao Q, Du ZP, Lv Z, Gu W, Pan F, Xu XE, Xie D, Li EM
The American journal of pathology 2010 Feb;176(2):939-51
The American journal of pathology 2010 Feb;176(2):939-51
Silencing cAMP-response element-binding protein (CREB) identifies CYR61 as a tumor suppressor gene in melanoma.
Dobroff AS, Wang H, Melnikova VO, Villares GJ, Zigler M, Huang L, Bar-Eli M
The Journal of biological chemistry 2009 Sep 18;284(38):26194-206
The Journal of biological chemistry 2009 Sep 18;284(38):26194-206
Roles of ezrin in the growth and invasiveness of esophageal squamous carcinoma cells.
Xie JJ, Xu LY, Xie YM, Zhang HH, Cai WJ, Zhou F, Shen ZY, Li EM
International journal of cancer 2009 Jun 1;124(11):2549-58
International journal of cancer 2009 Jun 1;124(11):2549-58
Premenstrual regulation of the pro-angiogenic factor CYR61 in human endometrium.
Gashaw I, Stiller S, Böing C, Kimmig R, Winterhager E
Endocrinology 2008 May;149(5):2261-9
Endocrinology 2008 May;149(5):2261-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cyr61/CCN1 Antibody [NB100-356] - Image shows a specific band for CYR61 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-356. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CYR61 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the low expression level of CYR61 in HepG2 cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.
