MA5-24187

antibody from Invitrogen Antibodies

Targeting: NMBR BB1, BB1R

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24187

Provider

Invitrogen Antibodies

Product name

NMBR Monoclonal Antibody (466505)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody stains human Neuromedin B R/NMBR transfectants but not irrelevant transfectants.

Antibody clone number

466505

Concentration

0.5 mg/mL

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HT-29 human colon adenocarcinoma cell line was stained with mouse Anti-human Neuromedin B R/NMBR Monoclonal Antibody (Product # MA5-24187) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NMBR in HEK293 human embryonic kidney cell line transfected with either (A) Neuromedin B R/NMBR or (B) irrelevant protein and eGFP. Samples were incubated in NMBR monoclonal antibody (Product # MA5-24187) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. Quadrant markers were set based on control antibody staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NMBR in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in NMBR monoclonal antibody (Product # MA5-24187) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.