GTX107327

antibody from GeneTex

Targeting: GTF2B TFIIB

Immunocytochemistry

Antibody data

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Validation data

GTF2B protein structure - GTX107327 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g whole cell lysate)A:H129910% SDS PAGEGTX107327 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HeLa whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HeLa whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HeLa whole cell and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500 and competitor's antibody (sc-274) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HeLa whole cell and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500 and competitor's antibody (sc-274) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.