Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX12094 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX12094, RRID:AB_370746
- Product name
- TFIIB antibody [IIB1]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa (lane 1), Jurkat (lane 2), and Raw264.7 (lane 3). Anti-TFIIB antibody (clone IIB1) was used, followed by HRP anti-mouse IgG antibody. £]-actin antibody was used as a loading control.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 £gg/ml anti-TFIIB (clone IIB1) in blocking buffer overnight at 4¢XC and followed by DyLight? 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa FluorR 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
