Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-49981 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49981, RRID:AB_10012171
- Product name
- Rabbit Polyclonal TFIIB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TFIIB Antibody [NBP1-49981] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. TFIIB was also immunoprecipitated by rabbit anti- GTF2B/ TFIIB antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.