AF2559

antibody from R&D Systems

Targeting: TGFBI BIGH3, CDB1, CDGG1, CSD1, CSD2, CSD3, LCD1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2559

Provider

R&D Systems

Product name

Mouse beta IG-H3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse beta IG-H3 in ELISAs and Western blots. In sandwich immunoassays, less than 6% cross-reactivity with recombinant human (rh) beta IG-H3 is observed.

Reactivity

Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P82198


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

beta IG-H3 in Mouse Embryo. beta IG-H3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse beta IG-H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2559) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.