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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-115322 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPSM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GPSM2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4(+) T-cell migration in SLE.
Esser RL, Brück C, Thiele J, Golumba-Nagy V, Meyer A, Steinbach-Knödgen E, Yan S, Tho Pesch C, Stahl D, Schiller J, Kofler DM
Lupus science & medicine 2022 Aug;9(1)
Lupus science & medicine 2022 Aug;9(1)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of GPSM2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115322) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
