PA5-18646

antibody from Invitrogen Antibodies

Targeting: GPSM2 DFNB82, LGN, Pins

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18646

Provider

Invitrogen Antibodies

Product name

GPSM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Kindt KS, Akturk A, Jarysta A, Day M, Beirl A, Flonard M, Tarchini B
Nature communications 2021 May 17;12(1):2861

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GPSM2 using GPSM2 Polyclonal Antibody (Product # PA5-18646) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GPSM2 in human testis. Samples were incubated with GPSM2 polyclonal antibody (Product # PA5-18646) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 GPR156-Galphai and GPSM2-Galphai are distinct functional modules in developing hair cells. a , b Normal apical enrichment of GPSM2-Galphai3 ( a ) or Galphai3 ( b ) in P1 Gpr156 mutant HCs in the cochlea ( a ) and utricle ( b ). Arrows indicate HC orientation. Note how GPSM2-Galphai3 enrichment follows HC orientation when HCs are inverted (OHC1-2s in a ) or fail to reverse orientation (lateral HCs in b ). Bottom panels show a magnified view of the HC boxed in the upper panels, and arrowheads point to GPSM2-Galphai3 at stereocilia tips ( a ). See Supplementary Fig. 7a for saccule results. c Scanning electron microscopy views of OHC1s (top) and IHCs (bottom) at 3 week (wk). IHC stereocilia stunting is obvious in PTXa (arrowhead) but not Gpr156 mutants. See Supplementary Fig. 7b for larger field views. d , e Auditory brainstem response (ABR, d ) and distortion product otoacoustic emissions (DPOAEs, e ) thresholds at ~4 week of age. X axis indicates broadband (click) and pure tone stimuli for ABR ( d ), or f2 for 2f1-f2 emission for DPOAEs ( e ). Y axis indicates the threshold of sound pressure level eliciting a response ( d ) or generating DPOAEs above noise floor ( e ) (mean +- SD; N indicates the number of animals tested per genotype; ABR click: Mann-Whitney test (two-tailed), **** p < 0.0001, ns p = 0.4943; ABR pure tones: two-way ANOVA with Sidak's multiple comparisons, **** p < 0.0001, ns is p = 0.6928 (8 kHz), p = 0.9167 (16 kHz), p = 0.1061 (32 kHz); DPOAE: two-way