Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- GTX104648 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104648, RRID:AB_1240770
- Product name
- Laforin antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Laforin, a dual specificity phosphatase involved in Lafora disease, is present mainly as monomeric form with full phosphatase activity.
Dukhande VV, Rogers DM, Romá-Mateo C, Donderis J, Marina A, Taylor AO, Sanz P, Gentry MS
PloS one 2011;6(8):e24040
PloS one 2011;6(8):e24040
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:Raji(GTX27908)12% SDS PAGEGTX104648 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody (GTX104648) diluted by 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Laforin antibody [C2C3], C-term detects EPM2A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: EPM2A protein stained by Laforin antibody [C2C3], C-term (GTX104648) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Laforin(GTX104648) antibody at 1:100 dilution.