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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-28073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPM2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EPM2A Polyclonal Antibody (Product # PA5-28073). Sample (30 µg of whole cell lysate). A: Raji. 12% SDS PAGE. EPM2A Polyclonal Antibody (Product # PA5-28073) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody EPM2A Polyclonal Antibody (Product # PA5-28073) diluted by 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EPM2A Polyclonal Antibody detects EPM2A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EPM2A protein stained by EPM2A Polyclonal Antibody (Product # PA5-28073) diluted at 1:500. Blue: Hoechst 33343 staining.
