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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA023034 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA023034, RRID:AB_1855095
- Product name
- Anti-PCYT2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human PCYT2, Gene description: phosphate cytidylyltransferase 2, ethanolamine, Alternative Gene Names: ET, Validated applications: WB, IHC, ICC, Uniprot ID: Q99447, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.8 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
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Enhanced validation
- Submitted by
- 57e50c6745390
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Orthogonal validation by targeted mass spectrometry with stable isotope labeled antigen (QPrEST). Target protein was quantified across eight cell lines and the same cell lysate was subjected for WB-analysis.
- Sample type
- Cell lysates
- Validation comment
- Single western blot band of expected molecular weight that shows excellent correlation if compared to quantitative data determined by MS.
- Primary Ab dilution
- 0.2 ug/ml
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:4000
- Protocol
- Protocol
- Correlation
- 1
- p-value
- 0.001
- Number of samples
- 8
