HPA025731

antibody from Atlas Antibodies

Targeting: RAB32

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA025731

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA025731, RRID:AB_1856001

Product name

Anti-RAB32

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIE
EAARFLVEKILVNHQSFPNEENDVDKIKLDQETLR
AENKSQCC


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-RAB32 antibody. Corresponding RAB32 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SK-MEL-30.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity with granular pattern in cortical cells.

Sample type

HUMAN