MA5-27674

antibody from Invitrogen Antibodies

Targeting: LRP4 CLSS, LRP-4, MEGF7, SOST2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27674

Provider

Invitrogen Antibodies

Product name

Anti-LRP4 Monoclonal Antibody (S164-6)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S164-6

Vial size

100 µg

Concentration

1 mg/ml

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRP4 in rat brain membrane with 10 µg of sample. The sample was incubated with LRP4 monoclonal antibody (Product # MA5-27674) using a dilution of 1:1000 (1 hour at RT), and followed by Goat Anti-Mouse (1 hour at RT) at a dilution of 1:200 and TMB development (10 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Rat Brain membrane.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LRP4 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with LRP4 monoclonal antibody (Product # MA5-27674) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) LRP4 (Cytoplasmic) Antibody, D) Merged image. Magnification: 60x.