PA5-30086

antibody from Invitrogen Antibodies

Targeting: MAFG MGC13090, MGC20149

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30086

Provider

Invitrogen Antibodies

Product name

MAFG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse muscle, rat muscle. Predicted reactivity: Mouse (98%), Rat (98%), Chicken (92%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.53 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MAFG using 30 µg of HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAFG polyclonal antibody (Product # PA5-30086) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MAFG was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFG Polyclonal Antibody (Product # PA5-30086) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MAFG Polyclonal Antibody (Product # PA5-30086). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG Polyclonal Antibody (Product # PA5-30086) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MAFG Polyclonal Antibody detects MAFG protein by western blot analysis. A.50 µg mouse muscle lysate/extract.12% SDS-PAGE. MAFG Polyclonal Antibody (Product # PA5-30086) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MAFG Polyclonal Antibody detects MAFG protein by western blot analysis. A.50 µg rat muscle lysate/extract.12% SDS-PAGE. MAFG Polyclonal Antibody (Product # PA5-30086) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MAFG was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG Polyclonal Antibody (Product # PA5-30086) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG (Product # PA5-30086) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MAFG was performed in HeLa whole cell extracts using 5 µg of MAFG Polyclonal Antibody (Product # PA5-30086). Samples were transferred to a membrane and probed with MAFG Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.