PA5-96695

antibody from Invitrogen Antibodies

Targeting: ASPSCR1 ASPL, ASPS, TUG, UBXD9, UBXN9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96695

Provider

Invitrogen Antibodies

Product name

Tug Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KSKSGQDPQQ EQEQERERDP QQEQERERPV DREPVDREPV VCHPDLEERL QAWPAELPDE FFELTVDDVR RRLAQLKSER KRLEEAPLVT KAFREAQIKE KLERYPKVAL RVLFPDRYVL QGFFRPSETV GDLRDFVRSH LGNPELSFYL FITPPKTVLD DHTQTLFQAN LFPAALVHLG AEEPAGVYLE PGLLEHAISP SAADVLVARY MSRAAGSPSP LPAPDPAPKS EPAAEEGALV PPEPIPGTAQ PVKRSLGKVP KWLKLPASKR; Positive Samples: 293T, SW480, A-549, BT-474, Mouse liver, Mouse testis; Cellular Location: Cytoplasm, Endomembrane system, Endoplasmic reticulum-Golgi intermediate compartment membrane, Nucleus, Peripheral membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.88 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Tug in extracts of various cell lines using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Tug was performed in HeLa cells using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Tug was performed in NIH/3T3 cells using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Tug was performed in PC-12 cells using Tug Polyclonal Antibody (Product # PA5-96695).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Tug in paraffin-embedded rat kidney using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Tug in paraffin-embedded human kidney cancer using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Tug in paraffin-embedded mouse lung using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Tug in paraffin-embedded mouse kidney using Tug Polyclonal Antibody (Product # PA5-96695) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Tug was performed in 200 µg extracts of A-549 cells using Tug Polyclonal Antibody (Product # PA5-96695). Western blot was performed from the immunoprecipitate using Tug Polyclonal Antibody.