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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324400 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal LRRC45 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal LRRC45 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LRRC45
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody (C-term)(Cat. #TA324400).TA324400 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using LRRC45 Antibody (C-term) (Cat. #TA324400). TA324400 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor?? 554 (red) conjugated Phalloidin (red).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LRRC45 Antibody (C-term) (Cat. #TA324400)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRRC45 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LRRC45 Antibody (C-term) (Cat. #TA324400) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
