Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
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Validation data
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- Product number
- NBP1-28904AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Lgr5/GPR49 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (M2-7C10) NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on SH-SY5Y cells with Lgr5/GPR49 Antibody [13A9] NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on HepG2 cells with Lgr5/GPR49 Antibody NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.