Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB15976 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- LGR5 (GPR49) antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (Cat.# OAAB15976) in K562 cell line lysates (35µg/lane). LGR5/GPR49(arrow) was detected using the purified Pab (1:60dilution).
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (Cat.# OAAB15976) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- LGR5 (GPR49) Antibody (Center) (Cat. # OAAB15976) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.