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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP2745f - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2745f, RRID:AB_2135153
- Product name
- LGR5 (GPR49) Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Multiple roles of integrin-linked kinase in epidermal development, maturation and pigmentation revealed by molecular profiling.
LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
Judah D, Rudkouskaya A, Wilson R, Carter DE, Dagnino L
PloS one 2012;7(5):e36704
PloS one 2012;7(5):e36704
LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines.
Walker F, Zhang HH, Odorizzi A, Burgess AW
PloS one 2011;6(7):e22733
PloS one 2011;6(7):e22733
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (Cat.#AP2745f) in K562 cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- LGR5/GPR49 Antibody (Center) (Cat.#AP2745f) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (Cat.#AP2745f), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- LGR5 (GPR49) Antibody (Center) (Cat. #AP2745f) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
