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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Flow cytometry [1]
- Other assay [1]
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- Product number
- MAB8078 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lgr5/GPR49 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Activin A-mediated epithelial de-differentiation contributes to injury repair in an in vitro gastrointestinal reflux model.
Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells.
Roudebush C, Catala-Valentin A, Andl T, Le Bras GF, Andl CD
Cytokine 2019 Nov;123:154782
Cytokine 2019 Nov;123:154782
Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells.
Chiou GY, Yang TW, Huang CC, Tang CY, Yen JY, Tsai MC, Chen HY, Fadhilah N, Lin CC, Jong YJ
Scientific reports 2017 May 19;7(1):2172
Scientific reports 2017 May 19;7(1):2172
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Human Lgr5/GPR49 Antibody Induces Activity. Human Lgr5/GPR49 Antibody Induces Activity.
