Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- GTX129862 - Provider product page
- Provider
- GeneTex
- Product name
- LGR5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells.
Terasaki M, Mima M, Kudoh S, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Oncology reports 2018 Jul;40(1):414-424
Oncology reports 2018 Jul;40(1):414-424
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LGR5 antibody detects LGR5 protein by western blot analysis.293T mock transfected (-) or transfected with a construct expressing human LGR5.7.5 % SDS-PAGELGR5 antibody (GTX129862) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LGR5 antibody detects LGR5 protein by western blot analysis. Non-transfected (-, unboiled) and LGR5-transfected (+, unboiled) 293T whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX129862) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) unboiled 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX129862) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.