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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA400001 - Provider product page
- Provider
- OriGene
- Product name
- Anti-LGR5 mouse mAb, clone OTI2A2, PE conjugated
- Antibody type
- Monoclonal
- Description
- Anti-LGR5 mouse mAb, clone OTI2A2, PE conjugated
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LGR5
- Isotype
- IgG
- Antibody clone number
- OTI2A2
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
Submitted references RSPO2 enriches LGR5(+) spheroid colon cancer stem cells and promotes its metastasis by epithelial-mesenchymal transition.
A human colonic crypt culture system to study regulation of stem cell-driven tissue renewal and physiological function.
Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures.
B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
The use of murine-derived fundic organoids in studies of gastric physiology.
Zhang S, Han X, Wei B, Fang J, Wei H
American journal of translational research 2016 Feb 15;8(2):354-64
American journal of translational research 2016 Feb 15;8(2):354-64
A human colonic crypt culture system to study regulation of stem cell-driven tissue renewal and physiological function.
Parris A, Williams MR
Methods in molecular biology (Clifton, N.J.) 2015;1212:141-61
Methods in molecular biology (Clifton, N.J.) 2015;1212:141-61
Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures.
Fraveto A, Cardinale V, Bragazzi MC, Giuliante F, De Rose AM, Grazi GL, Napoletano C, Semeraro R, Lustri AM, Costantini D, Nevi L, Di Matteo S, Renzi A, Carpino G, Gaudio E, Alvaro D
PloS one 2015 Nov 16;10(11):e0142124
PloS one 2015 Nov 16;10(11):e0142124
B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
Yang Y, Wu KE, Zhao E, Li W, Shi L, Xie G, Jiang B, Wang Y, Li R, Zhang P, Shuai X, Wang G, Tao K
Oncology letters 2015 Apr;9(4):1833-1838
Oncology letters 2015 Apr;9(4):1833-1838
The use of murine-derived fundic organoids in studies of gastric physiology.
Schumacher MA, Aihara E, Feng R, Engevik A, Shroyer NF, Ottemann KM, Worrell RT, Montrose MH, Shivdasani RA, Zavros Y
The Journal of physiology 2015 Apr 15;593(8):1809-27
The Journal of physiology 2015 Apr 15;593(8):1809-27
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry analysis of stem cell marker LGR5 by using anti-LGR5 antibody in native human colonic crypts microdissected from a fixed biopsy specimen.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HT29 cells are fixed with 2% formaldehyde for 20 min at RT, then stained with PE-labeled 2A2 anti-Lgr5 antibody. Black line: isotope control; Red line: anti-Lgr5-PE.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Flow cytometric analysis of gated cells co-expressing CD44 and LGR5 using FITC anti-CD44 antibody and anti-LGR5 PE conjugated antibody (TA400001). Dilution: 1:50
- Validation comment
- FC
